Activation of caspase-3/7, an apoptotic-related marker, during incubation and cryopreservation of alpaca (Vicugna pacos) spermatozoa

Simple item page
dc.contributor.authorSegura, Carlos
dc.contributor.authorLa Rosa, José
dc.contributor.authorBáez, Luis
dc.contributor.authorGómez Quispe, Oscar E.
dc.contributor.authorEvangelista Vargas, Shirley
dc.contributor.authorMorrell, Jane M.
dc.contributor.authorSantiani, Alexei
dc.date.accessioned2026-03-12T22:57:15Z
dc.date.available2026-03-12T22:57:15Z
dc.date.issued2026-03-12
dc.description.abstractCaspases are crucial mediators of programmed cell death (apoptosis). Apoptosis can occur in spermatozoa during spermatogenesis or epididymal transit, as well as in ejaculated spermatozoa. A high proportion of apoptotic sperm would be a poor indicator of the freezability of a raw seminal sample. Alpaca spermatozoa are notoriously difficult to freeze successfully. Therefore, the objectives of this study were to study caspase activation during incubation (37°C) of fresh alpaca spermatozoa, as well as before and after cryopreservation, to gain some insight into the mechanisms behind the vulnerability of alpaca spermatozoa. Eleven sperm samples were incubated for 4 h at 37°C (Study 1), and 23 samples were frozen using an automated system (Study 2). Caspase-3/7 activation was assessed at 0,1,2,3, and 4 h in samples incubated at 37°C (Study 1); and before/after cryopreservation (Study 2) using CellEvent™ Caspase 3/7 Green Detection Reagent and flow cytometry. The proportions of alpaca spermatozoa with caspase-3/7 activated increased (p < 0.05) after 3–4 h of incubation at 37°C; however, caspase activation was similar before and after cryopreservation (36.2 ± 11.2% vs. 36.6 ± 33.7%, p > 0.05). The high standard deviation found after freezing could be explained by the existence of two subpopulations: one subpopulation where caspase-3/7 activation decreased during cryopreservation (from 36.6 ± 9.1% to 1.5 ± 2.2%), and the other subpopulation where caspase-3/7 activation increased after cryopreservation (from 37.7 ± 13.0% to 64.3 ± 16.7%). In conclusion, after 3–4 h of incubation, caspase-3/7 activation increased in fresh alpaca sperm, whereas cryopreservation affects alpaca sperm samples in different ways.
dc.description.sponsorshipUniversidad Nacional Mayor de San Marcos, UNMSM; Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica, CONCYTEC VRIP‐UNMSM under grant A18081081‐2018 and by PROCIENCIA under grant 135‐2020‐FONDECYT
dc.formatapplication/pdf
dc.identifier.doihttps://doi.org/10.1111/rda.14397
dc.identifier.urihttps://hdl.handle.net/20.500.14195/1510
dc.language.isoeng
dc.publisherReproduction in Domestic Animals
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectAlpaca
dc.subjectApoptosis
dc.subjectCaspase
dc.subjectCryopreservation
dc.subjectSpermatozoa
dc.subject.ocdehttps://purl.org/pe-repo/ocde/ford#4.03.00
dc.titleActivation of caspase-3/7, an apoptotic-related marker, during incubation and cryopreservation of alpaca (Vicugna pacos) spermatozoa
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
A-Segura-Carlos.pdf
Size:
173.66 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.02 KB
Format:
Item-specific license agreed to upon submission
Description:
Download

Collections

Artículos Publicados