Detection and Isolation of Escherichia coli O157:H7 in Beef from Food Markets and Fecal Samples of Dairy Calves in the Peruvian Central Highlands

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dc.contributor.authorGonzales, Brenda L.
dc.contributor.authorAndrade, Daniel A.
dc.contributor.authorValdivia, Cesar A.
dc.contributor.authorHo-Palma, Ana C.
dc.contributor.authorMunguia, Astrid
dc.contributor.authorYucra, Dora
dc.contributor.authorEscobedo, Max
dc.contributor.authorCrotta, Matteo
dc.contributor.authorLimon, Georgina
dc.contributor.authorGonzalez, Armando
dc.contributor.authorGuitian, Javier
dc.contributor.authorGonzales Gustavson, Eloy
dc.date.accessioned2026-03-12T02:22:09Z
dc.date.available2026-03-12T02:22:09Z
dc.date.issued2026-03-12
dc.description.abstractShiga toxin-producing Escherichia coli (STEC) O157:H7 is a food and waterborne pathogen with severe public health implications. We report the first-time isolation of this pathogen in the Central Highlands of Peru through standardized culture procedures and polymerase chain reaction (PCR). Escherichia coli strains were cultured from rectal-anal swabs from dairy calves and beef from food markets. The latex agglutination test was used to detect O157 and H7 antigens, and multiplex real-time PCR was carried out to detect virulence-related genes. The STEC O157:H7 strains were isolated from 3.5% (1/28) of beef samples and from 6.0% (3/50) of dairy calves that also carried both eaeA and stx1 genes. Therefore, this pathogen is a potential cause of food/waterborne disease in the region, and its surveillance in both livestock and their products should be improved to characterize the impact of its zoonotic transmission. From 2010 to 2020, E. coli was suspected in 10 outbreaks reported to the Peruvian Ministry of Health. Isolates from future outbreaks should be characterized to assess the burden posed by STEC O157:H7 in Peru.
dc.description.sponsorshipConcytec/ProCiencia (Reference 0247-2019-PROCIENCIA)
dc.formatapplication/pdf
dc.identifier.doihttps://doi.org/10.4269/ajtmh.23-0181
dc.identifier.urihttps://hdl.handle.net/20.500.14195/1484
dc.language.isoeng
dc.publisherThe American Journal of Tropical Medicine and Hygiene
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectArticle
dc.subject.ocdehttps://purl.org/pe-repo/ocde/ford#4.03.00
dc.titleDetection and Isolation of Escherichia coli O157:H7 in Beef from Food Markets and Fecal Samples of Dairy Calves in the Peruvian Central Highlands
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion

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